When picking needle gauge sizes it is vital to keep in psychic the volume of the syringe and also the dead volume that the needle. Because that example, it will be very complicated to element a 10 µL syringe if the dead volume in the needle is greater than 10 µL. Describe the needle gauge graph to choose a needle gauge through an ideal µL/inch prior to selecting a needle. Pick the minimum needle length that allows you to carry out your applications comfortably.

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Note: The ‘s’ ~ above a 22s needle to represent a smaller I.D. (inner diameter) because that the needle and a more thickness needle wall surface for better durability. For example, a 26 gauge needle has an O.D. (outer diameter) the 0.46 mm and an I.D. Of 0.26 mm when the 26s gauge needle has an O.D. That 0.47 mm and an I.D. That 0.13 mm. The 26s has half the I.D. The the 26 gauge needle. Also, the difference in the wall surface thickness almost doubles v 26s gauge having actually a thickness the 0.18 mm when the 26 gauge is just 0.10 mm.

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Gauge NumberNeedle in the name O.D.(mm)Needle in the name of I.D.(mm)Needle wall surface Thickness(mm)Needle Dead Volume(µL/25.4 mm)
34 gauge needle 0.159 0.051 0.051 0.052 µL/25.4 mm
33 gauge needle 0.21 0.108 0.051 0.233 µL/25.4 mm
32 gauge needle 0.235 0.108 0.064 0.233 µL/25.4 mm
31 gauge needle 0.261 0.133 0.064 0.353 µL/25.4 mm
30 gauge needle 0.312 0.159 0.076 0.504 µL/25.4 mm
29 gauge needle 0.337 0.184 0.076 0.675 µL/25.4mm
28 gauge needle 0.362 0.184 0.089 0.675 µL/25.4 mm
27 gauge needle 0.413 0.21 0.102 0.876 µL/25.4 mm
26s gauge needle 0.474 0.127 0.178 0.322 µL/25.4 mm
26 gauge needle 0.464 0.26 0.102 1.349 µL/25.4 mm
25s gauge needle 0.515 0.153 0.178 0.464 µL/25.4 mm
25 gauge needle 0.515 0.26 0.127 1.349 µL/25.4 mm
24 gauge needle 0.566 0.311 0.127 1.93 µL/25.4 mm
23s gauge needle 0.642 0.116 0.267 0.268 µL/25.4 mm
23 gauge needle 0.642 0.337 0.152 2.266 µL/25.4 mm
22s gauge needle 0.718 0.168 0.279 0.563 µL/25.4 mm
22 gauge needle 0.718 0.413 0.152 3.403 µL/25.4 mm
21 gauge needle 0.819 0.514 0.152 5.271 µL/25.4 mm
20 gauge needle 0.908 0.603 0.152 7.255 µL/25.4 mm
19 gauge needle 1.067 0.686 0.191 9.389 µL/25.4 mm
18 gauge needle 1.27 0.838 0.216 14.011 µL/25.4 mm
17 gauge needle 1.473 1.067 0.203 22.715 µL/25.4 mm
16 gauge needle 1.651 1.194 0.229 28.444 µL/25.4 mm
15 gauge needle 1.829 1.372 0.229 37.529 µL/25.4 mm
14 gauge needle 2.109 1.6 0.254 51.076 µL/25.4 mm
13 gauge needle 2.413 1.804 0.305 64.895 µL/25.4 mm
12 gauge needle 2.769 2.159 0.305 93 µL/25.4 mm
11 gauge needle 3.048 2.388 0.33 113.728 µL/25.4 mm
10 gauge needle 3.404 2.693 0.356 144.641 µL/25.4 mm
*mm are nominal

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